2DD-100SBRP

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2DD-100SBRP is an electrophoresis system. It is a powerful instrument for purifying macromolecules like proteins, DNA, and RNA. It is used in various research laboratories and biopharmaceutical companies. It has a wide application field and offers high performance and reliability.

The application field of 2DD-100SBRP mainly includes in-gel and out-gel electrophoresis, protein purification, DNA sequencing, and other related applications. The system also offers a wide range of features such as a variety of buffer modules, sample preparation protocols, large sample capacity, and powerful data analysis capabilities. In-gel and Out-gel electrophoresis are two common types of methods used in protein purification. In gel electrophoresis is used to separate charged molecules in a solution using a gel matrix. Whereas, out-gel electrophoresis is a technique used for purifying large molecules, such as proteins, from a sample. It uses a membrane-like matrix and electric current to move the molecules across the membrane.

The working principle of 2DD-100SBRP relies on applying a voltage across an electrolytic medium, such as a gel matrix or a membrane. To perform electrophoresis, proteins, DNA, or RNA molecules are applied on a medium, and then a high voltage (typically in the range of 500-1000V) is applied across the medium. This causes the electric field to draw the molecules towards the oppositely charged electrode.

The principle employed by 2DD-100SBRP has a few important components. Firstly, it is important to choose an appropriate buffer for the application. Buffers are a solution in which charged particles remain in the medium, even when a voltage is applied. Without the presence of the buffer, the electric current could damage the molecules in the sample. The buffer must also be suitable for the specific type of molecule that is being separated.

Secondly, the voltages used must be appropriate for the specific molecules being tested. If the voltage is too high, it will cause the molecules to become damaged, and they will not migrate properly. On the other hand, if the voltage is too low, the separation speed of the molecules will be too slow.

Once the electric current starts flowing, it causes the molecules in the sample to move in the direction of the oppositely charged electrode. This process is known as “field-driven” migration, which is enabled by the differences in electrical charge between the different molecules in the sample. The more positive molecules move towards the negative electrode, and the more negative molecules move towards the positive electrode. Different molecules will move at different speeds depending on their charge and size.

The separation of molecules that occurs during electrophoresis can be further enhanced by using drugs that bind to specific molecules. This is known as “affinity electrophoresis.” The drugs allow certain molecules to bind to them and move in the same direction, while others will remain unbound and move at different speeds. This technique can be used to separate proteins, DNA, and RNA molecules.

In conclusion, the 2DD-100SBRP offers a variety of features and applications, and its working principle is based on the principles of electrophoresis. This system is widely used in laboratories and biopharmaceutical companies to purify macromolecules like proteins, DNA, and RNA. It is a powerful tool for researchers, as it offers high performance and reliability.

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