CFG2EI

CFG2EI, or chromogenic fluorescence-based gene expression in single-cells, is a method of detecting and measuring gene expression in single cells. It is an exciting advancement in the field of gene expression because it allows researchers to capture the gene expression activity of individual cells in a sample, rather than in a group of cells. This makes it possible to identify gene expression patterns that couldn\'t be seen before in groups of cells.

The CFG2EI application field includes single cell level monitoring of gene expression, multiplex imaging in complex cell environments, detailed molecular analysis of tissue samples, study of epigenetics in single cells, and understanding of gene regulatory networks. It can also provide molecular insight into the mechanistic basis of cellular decisions and behaviour.

The basic working principle of CFG2EI is that fluorescent probes are used to detect and measure gene expression. This process involves two steps: transcription and fluorescent emission. During the transcription step, a cell\'s messenger RNA (mRNA) is extracted, reverse transcribed into complementary DNA (cDNA) and hybridized to later identify and quantify the expression of a certain gene. The second step involves the fluorescent emission of the probes, which are enzymes that catalyze the oxidation of a reporter molecule to give off a fluorescent signal.

The reporter molecules are typically tetramethylrhodamine (TMR), thiazole orange (TO), or Cy3. The reporter molecule undergoes oxidation in the presence of the enzyme, in this case, the reporter molecule forms a fluorophore, which absorbs light of a certain wavelength and is then fluorescently emitted. This detection is made possible by the use of flow cytometry which detect and gather the fluorescent light signals coming from the reporter molecules.

The advantage of CFG2EI lies in its sensitivity and specificity. It enables researchers to accurately measure gene expression in individual cells with minimal background noise. This means researchers can more accurately and reliably measure gene expression and study the individual cell with greater detail. Additionally, the versatility of the method allows for single-cell expression profiling in a variety of cell types, including normal and tumor cells. The method has also been used to accurately measure expression levels in many organisms including bacteria, fungi, plants and animals.

Overall, CFG2EI has enabled researchers to gain a greater understanding of gene regulation in individual cells and how gene expression can influence cell behaviour and decisions. It is a powerful method for studying gene expression at the single cell level and can provide valuable insights into gene regulatory networks. By leveraging the concept of fluorescence-based gene expression in single cells, researchers can gain a deeper understanding of genes and how they are regulated in complex cellular systems.