CJRCAPRD

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CJRCAPRD, which stands for Cell Joint Receptor Proteomics Array Platform with Reagent Dispensing, is a novel platform developed to measure protein interaction networks in cells. This technology takes advantage of a combination of cell-based protein array and microfluidic technologies to create an automated, disposable, and portable system for rapid and simultaneous detailed protein interaction analyses in adherent cell lines.

The CJRCAPRD platform is composed of several components, including a microfluidic chip, a microscope, a detector, a detector receiver, a PC, and a reagent dispenser. The microfluidic chip is made from an array of troughs, or “channels”, that are overlaid with a specially prepared protein array. This protein array contains an array of protein molecules attached to an adhesive medium, such as a nutrient gel or an agarose bead. The protein molecules act as immobilized capture ligands that bind specific target proteins present in the cell sample.

The cells are first placed onto the protein array and the microfluidic chip is then placed inside the detector. Using a specialized optical microscope, the detector is then able to detect the interactions between the target proteins and the immobilized capture ligands. The detector then sends a signal to the detector receiver, which sends the information to the PC to be processed and analyzed. The PC then sends the results to the reagent dispenser, which dispenses the detected target proteins into separate buffer solutions.

The CJRCAPRD platform has many advantages over traditional proteome profiling methods. Since the platform is completely automated, it can be used for high throughput analysis. Additionally, since the platform is portable, it can be used in any laboratory or field environment with minimal setup and calibration. Finally, as the platform relies on disposable and reusable components, it can significantly reduce labor costs as well as the amount of samples required for an analysis.

Overall, CJRCAPRD is an innovative platform that offers a rapid and high-throughput way to measure protein interactions in cells. This revolutionary technology provides a more cost-effective and accurate approach to proteomics research, enabling researchers to analyze more data with less effort.

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